学术报告
题目： Advanced Technology of Single Cell Gene Analysis
报告人： Peilin Chen
Fluidigm R&D Senior Scientist
时间：2013年5月9日（周四）上午10:30 AM
地点：Biopic大会议室
联系人：李瑞强
Biography
Peilin Chen, R&D scientist, received his Ph.D in 1989 from China Pharmaceutical University. His career started with his post doctoral training at College of Pharmacy, University of Kentucky, after his graduation. Now he has been working with Fluidigm Corporation as a Staff Applications Development Scientist since 2007 and is a key contributor in the development of both the Fluidigm Access Array and C1 Single Cell Auto Prep Systems. Before joining Fluidigm, he worked at Bio-Rad Laboratories as Senior Staff Scientist and Group Leader, developing in vitro diagnostics from 1999-2007. Before Bio-Rad, he worked with 3 other startup companies from 1994-1999 as a scientist and technical director.
Abstract: Single-cell gene expression profiling has recently been used to characterize emergent properties in cell populations that drive lineage choice and specificity in reprogrammed cells, decisions in very early embryonic development, and to identify cancer stem cell biomarkers in tumor biopsies. We have developed a simple, modular workflow for streamlined analysis of cell populations down to the single-cell level. The workflow is centered on two key components: the C1™ Single-Cell Auto Prep System for automated cell isolation and cDNA preparation, and the BioMark™ HD System for highly parallel gene expression analysis. Starting from samples containing only a few hundred cells, the preparation system isolates individual cells into discrete compartments within a microfluidic device. Cells can be inspected after isolation using either brightfield microscopy or fluorescent markers to verify cell number and type. Following cell inspection and verification, the selected individual cells are automatically processed to prepare and output high-quality targeted cDNA for gene expression analysis using quantitative PCR. Targeted cDNA samples are loaded and analyzed in parallel with up to 96 gene expression assays per cell on Dynamic Array™ IFC`s using the BioMark™ HD System. We are in the process of developing a preparation method for full length mRNA-sequencing libraries using the cell capture, isolation and imaging capabilities the C1 System. The ability to prepare mRNA-Seq libraries from single cells will enable detailed descriptions of transcription in cell populations at the level of the individual cell. Cells will be automatically processed into high-quality cDNA that can be used to prepare sequencing libraries for single-cell transcriptome analysis. Characterization of variations in gene expression within cell populations has revealed a wide spectrum of variation in gene expression and within signaling networks that drive the changes behind key cellular transitions in a variety of biological systems.
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