学术报告
题目：RNA pseudouridylation and 2`-O-methylation: new insights into two old modifications
报告人：Yitao Yu, Ph.D
Center for RNA Biology, University of Rochester Medical Center,
时间：2013年6月4日 (周二) 10:30‐11:30 AM
地点：生命科学学院金光生科大楼 311会议室
Pseudouridine and 2`-O-methylated nucleotides are the most abundant post-transcriptionally modified nucleotides in various stable RNAs of all organisms. While pseudouridine is derived from uridine via base-specific isomerization, resulting in an extra hydrogen-bond donor that distinguishes it from other nucleotides, 2`-O-methylated nucleotides are generated by a sugar-specific reaction in which a methyl group is added to the 2`-O position of a ribonucleotide. In eukaryotes, pseudouridylation and 2`-O-methylation are catalyzed primarily by box H/ACA RNPs and box C/D RNPs, respectively. Both box H/ACA RNPs and box C/D RNPs are ribonucleoproteins (RNA-protein complexes) that act as modifying enzymes, with the RNA component serving as a guide. When introduced into RNA, pseudouridine and 2`-O-methylated residues contribute significantly to RNA-mediated cellular processes. Recently, we discovered that pseudouridylation can be induced by stress, suggesting a regulatory role for this RNA modification. We also showed that pseudouridylation and 2`-O-methylation can be specifically introduced into a number of different types of RNA (target RNAs) by artificial box H/ACA RNPs and box C/D RNPs, and that such introductions can alter the functions of target RNAs. Our work provides new insights into the mechanisms and functions of RNA-guided ribonucleotide modifications.
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